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primer analyzer   Dan primer analyzer

Tool for analyzing and comparing multiple primer sequences simultaneously

Select the polymerase or kit from the list of products If needed, modify the recommended primer concentration Enter primer sequences (with up to 3 ambiguous Analyze → Primer3“ The „Primer Design“ dialog will be opened to design primers It has plenty of options, just like the web interface does, but eventually

ufa365k Xiaowei Wang, Athanasia Spandidos, Huajun Wang and Brian Seed: PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update  PCR Primer Designing NCBI Primer BLAST

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